Calendario Eventi

24 gennaio 2019


presso l'Aula Pacinotti dalle ore 15,30 alle ore 17,30




Brain micro-architecture:  from clarified tissues to single neuron isolation

Alejandro Luis Callara 

PhD candidate in the Dipartimento di Ingegneria dell'Informazione and in the Research Center "E.Piaggio" at the University of Pisa



A framework for the isolation of single neurons: integration of clarification methods and open source tools for image segmentation

Accurately mapping the brain at the micro-scale is still a challenge in cellular neuroscience. In recent years, new-tissue clearing techniques coupled with well-established microscopy imaging methods (e.g. confocal and two-photon microscopy) enabled to acquire volumetric data of the global arrangement of large brain neuron populations at the cellular resolution. On the other hand, several algorithms for automatic neuron segmentation aiming at obtaining accurate morphological and topological organization of neurons have been proposed. Notable success have been reach in both fields. However, their integration has been poorly conceived. As a result, an entire new class of clarified images are available, but algorithms purposely developed to deal with such images are still lacking. In this seminar, we will present a new framework for the analysis of neuron morphology, integrating the optimization of clarification methods with purposely developed open source tools for the segmentation of single neurons from clarified microscopy data.




Il seminario  è organizzato dal Centro di ricerca Enrico Piaggio


Direttore del Centro: prof.ssa Ahluwalia Arti Devi